MCM2 (D7G11) XP® Rabbit mAb【科瑞奥博】

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MCM2 (D7G11) XP® Rabbit mAb

货号： 3619T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	3619T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , IF-IC , ChIP
目标/特异性	MCM2 (D7G11) XP^® Rabbit mAb detects endogenous levels of total MCM2 protein.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:800) IF-IC (1:800) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2 through MCM7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and Cdt1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4 and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have also shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).微小染色体维持（MCM）蛋白2-7是DNA复制起始和延伸所需的6个相关蛋白家族。 MCM2到MCM7蛋白相互结合，形成异源六聚体MCM复合物，被认为充当了DNA复制叉的复制解旋酶（1-5）。该复合体也是前复制复合体(pre-RC)的关键组成部分(回顾1)。Cdc6 和 Cdt1招募MCM复合体至有丝分裂末期/G1早期的起始识别复合体(ORC)形成pre-RC，促进DNA复制(2中已论述)。MCM2, MCM3, MCM4和MCM6亚单位的磷酸化似乎调节MCM复合体的活性和DNA合成的启动(6-8)。DNA复制时MCM蛋白被清除，通过pre-RC重构的抑制引起染色质未识别化。染色质批准允许每个细胞循环DNA复制一次，有助于保证基因选择和避免恶性肿瘤细胞的产生(回顾3)。研究亦表明MCM复合体通过保护复制叉的结构参与检验点控制，而且可以招募捕获后的检验点蛋白协助复制重启(回顾3,9)。
存放说明	-20C
计算分子量	125
参考文献	1 . Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54. 2 . Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3. 3 . Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31. 4 . Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6. 5 . Labib, K. et al. (2000) Science 288, 1643-7. 6 . Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86. 7 . Masai, H. et al. (2006) J Biol Chem 281, 39249-61. 8 . Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84. 9 . Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72. 10 . Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes, and either 20 μl of MCM2 (D7G11) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human TAP1 Promoter Primers #5148, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀方法检测4x106 HT-1080细胞的交联染色质，细胞分别进行以下处理：IFN-γ (50 ng/ml)处理30分钟，加入20 μl of MCM2 (D7G11) XP® Rabbit mAb或者2 μl of Normal Rabbit IgG #2729，使用的试剂盒为 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集的DNA用real-time PCR技术定量，使用的引物为SimpleChIP® Human TAP1 Promoter Primers #5148，human IRF-1 promoter primers,，以及SimpleChIP® Human α Satellite Repeat Primers #4486。以input染色质总量为1，每个样品中IP的DNA量用相对于input 染色质的相对数表示。

Immunohistochemical analysis of paraffin-embedded human small intestine using MCM2 (D7G11) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋人类小肠组织，使用的抗体为MCM2 (D7G11) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MCM2 (D7G11) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋人类结肠癌组织，使用的抗体为MCM2 (D7G11) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MCM2 (D7G11) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋人类肺癌组织，使用的抗体为MCM2 (D7G11) XP® Rabbit mAb。

Confocal immunofluorescent analysis of HeLa cells using MCM2 (D7G11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测HeLa细胞，使用的抗体为MCM2 (D7G11) XP® Rabbit mAb ，呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。

Western blot analysis of extracts from various cell types using MCM2 (D7G11) XP® Rabbit mAb.western blot方法检测不同细胞提取物，使用的抗体为MCM2 (D7G11) XP® Rabbit mAb。

Immunoprecipitation of MCM2 from HeLa cell lysates using MCM2 (D7G11) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input. 从HeLa细胞裂解液中免疫沉淀MCM2，使用的抗体为 MCM2 (D7G11) XP® Rabbit mAb ，随后用同一抗体进行western blot实验。泳道1为5%对照。