ZO-3 (D57G7) XP® Rabbit mAb【科瑞奥博】

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ZO-3 (D57G7) XP® Rabbit mAb

货号： 3704T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	3704T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IF-IC
目标/特异性	ZO-3 (D57G7) XP® Rabbit mAb detects endogenous levels of total ZO-3 protein.
使用方法	WB(1:1000) IF-IC (1:1600)

性能
供应商	CST
灵敏度	Endogenous
背景	Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).
存放说明	-20C
计算分子量	140
参考文献	1 . Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 2 . Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11. 3 . Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47. 4 . Umeda, K. et al. (2006) Cell 126, 741-54. 5 . Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66. 6 . Traweger, A. et al. (2003) J Biol Chem 278, 2692-700. 7 . Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36. 8 . Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24. 9 . Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36. 10 . Adachi, M. et al. (2006) Mol Cell Biol 26, 9003-15. 11 . Xu, J. et al. (2008) Mol Cell Biol 28, 1669-78.

参考图片

Western blot analysis of extracts from MCF-7, HT-29 and HCT-15 cells using ZO-3 (D57G7) XP® Rabbit mAb.使用ZO-3 (D57G7) XP® 兔单抗对MCF-7, HT-29 和 HCT-15细胞提取物进行western blot分析。

Confocal immunofluorescent analysis of MCF-7 cells using ZO-3 (D57G7) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用ZO-3 (D57G7) XP®兔单抗（绿色）对MCF-7细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® #4084（DNA荧光染料）。