| 货号 | 3798T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P |
| 目标/特异性 | ERp44 (D17A6) XP® Rabbit mAb detects endogenous levels of total ERp44 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum resident protein 44 (ERp44), constitute the PDI family (2). ERp44 is induced upon ER stress and is linked to Ero1-Lα and Ero1-Lβ through mixed disulfide bonds (3). ERp44 was shown to mediate the ER localization of Ero1-Lα (4). |
| 存放说明 | -20C |
| 计算分子量 | 44 |
| 参考文献 | 1 . Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8. 2 . Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32. 3 . Anelli, T. et al. (2002) EMBO J 21, 835-44. 4 . Anelli, T. et al. (2003) EMBO J 22, 5015-22. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERp44 (D17A6) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人肺癌使用ERp44 (D17A6) XP® Rabbit mAb兔单抗进行免疫组织化学分析，其中左图加入对照肽，右图加入抗原特异肽。 | |
Western blot analysis of extracts from various cell types using ERp44 (D17A6) XP® Rabbit mAb. 对多种细胞系使用ERp44 (D17A6) XP® Rabbit mAb兔单抗进行Western blot分析。 |
