| 货号 | 3833T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | eIF6 (D16E9) XP® Rabbit mAb detects endogenous levels of total eIF6 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Sanvito, F. et al. (1999) J Cell Biol 144, 823-37. 2 . Ceci, M. et al. (2003) Nature 426, 579-84. 3 . Chendrimada, T.P. et al. (2007) Nature 447, 823-8. |
Confocal immunofluorescent analysis of HeLa cells using eIF6 (D16E9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用eIF6 (D16E9) XP® Rabbit mAb兔单抗 (绿色)，共聚焦免疫荧光观察eIF6蛋白在HeLa细胞中定位。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from various cell types using eIF6 (D16E9) XP® Rabbit mAb. 使用eIF6 (D16E9) XP® Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系提取物。 |
