| 货号 | 3868T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | LC3B (D11) XP® Rabbit mAb detects endogenous levels of total LC3B protein. Cross-reactivity may occur with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:3200) F (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
| 参考文献 | 1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4 . Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97. 5 . Lang, T. et al. (1998) EMBO J. 17, 3597-607. 6 . Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28. 7 . He, H. et al. (2003) J. Biol. Chem. 278, 29278-87. 8 . Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10. 9 . Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42. 10 . Ichimura, Y. et al. (2000) Nature 408, 488-92. 11 . Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® LC3B siRNA I #6212 (+) or SignalSilence® LC3B siRNA II #6213 (+), using LC3B (D11) XP® Rabbit mAb #3868 and α-Tubulin (11H10) Rabbit mAb #2125. The LC3B (D11) XP® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. Western blot分析HeLa细胞的提取物，分别用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® LC3B siRNA I #6212 (+)或SignalSilence® LC3B siRNA II #6213 (+)转染。使用的抗体是：LC3B (D11) XP® Rabbit mAb 兔单抗#3868 和 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。α-Tubulin (11H10) Rabbit mAb 兔单抗作为加样和LC3B siRNA的特异性对照，LC3B (D11) XP® Rabbit mAb 兔单抗证实LC3B 表达的沉默。 | |
Immunohistochemical analysis of paraffin-embedded human astrocytoma using LC3B (D11) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分析石蜡包埋的人星形细胞瘤，使用的抗体是LC3B (D11) XP® Rabbit mAb 兔单抗，分别经对照肽（左）或 抗原特异性多肽 （右）处理。 | |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or chloroquine-treated (right), using LC3B (D11) XP® Rabbit mAb. 免疫组化分析石蜡包埋的HeLa细胞沉淀，对照（左）或 chloroquine (氯喹)处理（右），使用的抗体是LC3B (D11) XP® Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of HeLa cells using LC3B (D11) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪分析HeLa细胞，使用的抗体是LC3B (D11) XP® Rabbit mAb 兔单抗（蓝色），一种非特异的阴性对照抗体（红色）作为对照。 | |
Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3B (D11) XP® Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines, untreated (-) or treated overnight with chloroquine (50 μM) (+), using LC3B (D11) XP® Rabbit mAb (upper) or LC3B Antibody #2775 (lower). Western blot分析不同细胞系的提取物，未处理(-)或chloroquine（氯喹）处理(50 μM,过夜，+)，使用的抗体分别是LC3B (D11) XP® Rabbit mAb 兔单抗（上图）和LC3B Antibody #2775（下图）。 |
