| 货号 | 13116T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | N-Cadherin (D4R1H) XP® Rabbit mAb recognizes endogenous levels of total N-cadherin protein. Some non-specific staining has been observed in mouse kidney tissue. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:125) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. 2 . Christofori, G. (2003) EMBO J 22, 2318-23. 3 . Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. 4 . Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34. 5 . Rabascio, C. et al. (2004) Cancer Res 64, 4373-7. 6 . Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7. 7 . Patel, I.S. et al. (2003) Int J Cancer 106, 172-7. 8 . Sanders, D.S. et al. (2000) J Pathol 190, 526-30. |
Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).使用N-Cadherin (D4R1H) XP® Rabbit mAb(绿色)对A172 (阳性，左)和MCF7 (阴性，右)进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye)。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.使用N-Cadherin (D4R1H) XP® Rabbit mAb对石蜡包埋的人卵巢癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.使用N-Cadherin (D4R1H) XP® Rabbit mAb对石蜡包埋的A172 (阳性，左)和MCF7 (阴性，右)进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus. 使用N-Cadherin (D4R1H) XP®Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。注意肌间神经丛的染色。 | |
Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).使用N-Cadherin (D4R1H) XP® Rabbit mAb (上) 或β-Actin (D6A8) Rabbit mAb #8457 (下)对A172和MCF7细胞提取物进行western blot分析。 |
