T7-Tag (D9E1X) XP® Rabbit mAb【科瑞奥博】

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T7-Tag (D9E1X) XP® Rabbit mAb

货号： 13246T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	13246T
同种亚型	Rabbit IgG
反应种属	All
来源宿主	Rabbit
应用	W/IP/IF-IC
目标/特异性	T7-Tag (D9E1X) XP^® Rabbit mAb recognizes transfected levels of recombinant protein containing the T7 epitope tag.
使用方法	WB(1:1000) IP (1:50) IF-IC (1:200)

性能
供应商	CST
灵敏度	Transfected Only
背景	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties.
存放说明	-20C
参考文献	1 . Studier, F.W. and Moffatt, B.A. (1986) J Mol Biol 189, 113-30.

参考图片

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing a T7-tagged GFP fusion protein (T7-GFP; +), using T7-Tag (D9E1X) XP^® Rabbit mAb.Western blot检测293T细胞提取物，未转染(-)或转染了表达T7标签GFP融合蛋白(T7-GFP; +)，使用的抗体是T7-Tag (D9E1X) XP® Rabbit mAb。

Confocal immunofluorescent analysis of 293T cells transiently transfected with a T7-tagged GFP protein (green) using T7-Tag (D9E1X) XP^® Rabbit mAb (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析293T细胞，用T7标记的GFP（绿色）融合蛋白瞬时转染，使用的抗体是：T7-Tag (D9E1X) XP® Rabbit mAb（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Immunoprecipitation of transfected T7-tagged GFP fusion protein from 293T cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or T7-Tag (D9E1X) XP^® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using T7-Tag (D9E1X) XP^® Rabbit mAb.对293T细胞提取物中的T7标签融合蛋白进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或T7-Tag (D9E1X) XP® Rabbit mAb (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是T7-Tag (D9E1X) XP® Rabbit mAb。