UCHL1 (D3T2E) XP® Rabbit mAb【科瑞奥博】

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UCHL1 (D3T2E) XP® Rabbit mAb

货号： 13179T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	13179T
同种亚型	Rabbit IgG
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IHC-P/IF-IC
目标/特异性	UCHL1 (D3T2E) XP^® Rabbit mAb recognizes endogenous levels of total UCHL1 protein. This antibody does not cross-react with other UCH family members.
使用方法	WB(1:1000) IHC-P (1:400) F (1:400) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the ubiquitin carboxy-terminal hydrolase (UCH) family of DUBs, which all possess a conserved catalytic UCH domain of about 230 amino acids. UCHL5 and BAP1 have unique, extended carboxy-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed (3,4). Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule.
存放说明	-20C
计算分子量	27
参考文献	1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Leroy, E. et al. (1998) Nature 395, 451-2. 4 . Kurihara, L.J. et al. (2001) Hum Mol Genet 10, 1963-70. 5 . Todi, S.V. and Paulson, H.L. (2011) Trends Neurosci 34, 370-82. 6 . Setsuie, R. and Wada, K. Neurochem Int 51, 105-11. 7 . Day, I.N. and Thompson, R.J. (2010) Prog Neurobiol 90, 327-62. 8 . Xiang, T. et al. (2012) PLoS One 7, e29783.

参考图片

Confocal immunofluorescent analysis of DU 145 (positive; left) and LNCaP (negative; right) cells using UCHL1 (D3T2E) XP^® Rabbit mAb (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).Confocal immunofluorescent检测DU 145 细胞(阳性; 左) 和LNCaP细胞（阴性；右），采用UCHL1 (D3T2E) XP® Rabbit mAb（绿色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Immunohistochemical analysis of paraffin-embedded DU 145 (left; positive) and LNCaP (right; negative) cell pellets using UCHL1 (D3T2E) XP^® Rabbit mAb.免疫组化分析石蜡包埋的DU 145（左；阳性）和LNCaP（右；阴性）细胞，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb.免疫组化分析石蜡包埋的人前列腺恶性瘤，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb.免疫组化分析石蜡包埋的人非小细胞肺癌，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).免疫组化分析石蜡包埋的人结直肠腺癌，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb，对照肽段（左）或抗原特异性肽段（右）。

Western blot analysis of extracts from various cell lines using UCHL1 (D3T2E) XP^® Rabbit mAb (upper) and GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower).Western blot检测多种细胞提取物，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb (上图)和GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UCHL1 (hUCHL1-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL3 (hUCHL3-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL5 (hUCHL5-Myc/DDK; +), and Myc/DDK-tagged full-length human BAP1 (hBAP1-Myc/DDK; +), using UCHL1 (D3T2E) XP^® Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).Western blot检测293T细胞提取物，未转染(-)或转染表达Myc/DDK标签人全长UCHL1 (hUCHL1-Myc/DDK; +)、Myc/DDK标签人全长UCHL3 (hUCHL3-Myc/DDK; +)、Myc/DDK标签人全长UCHL5 (hUCHL5-Myc/DDK; +)和Myc/DDK标签人全长BAP1 (hBAP1-Myc/DDK; +)，使用的抗体是UCHL1 (D3T2E) XP® Rabbit mAb (上图)或者DYKDDDDK Tag Antibody #2368 (下图)。

Flow cytometric analysis of K-562 cells (blue) and Ramos cells (green) using UCHL1 (D3T2E) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from Ramos and K-562 cells using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, UCHL1 protein expression is not detected in K-562 cells.