Notch1 (D6F11) XP® Rabbit mAb【科瑞奥博】

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Notch1 (D6F11) XP® Rabbit mAb

货号： 4380T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	4380T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,
来源宿主	Rabbit IgG
应用	WB, F , IF-IC
目标/特异性	Notch1 (D6F11) XP® Rabbit mAb detects intracellular epitopes between 2400 and 2500 amino acids of human Notch1. It recognizes both the full-length (~300 KDa) and the NTM region (~120 KDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.
使用方法	WB(1:1000) F (1:800) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
存放说明	-20C
计算分子量	120, 300
参考文献	1 . Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. 2 . Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. 3 . Schroeter, E.H. et al. (1998) Nature 393, 382-6. 4 . Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

参考图片

Confocal immunofluorescent analysis of MCF7 cells using Notch1 (D6F11) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

使用Notch1 (D6F11) XP^® Rabbit mAb (绿色)对MCF7细胞进行激光共聚焦荧光分析。蓝色伪彩=DRAQ5^® #4084 (DNA荧光染料).

Flow cytometric analysis of Jurkat cells using Notch1 (D6F11) XP^® Rabbit Ab (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

使用Notch1 (D6F11) XP^® Rabbit Ab （蓝色）与相应浓度的Rabbit (DA1E) mAb IgG XP^®同型对照 #3900 （红色）对Jurkat细胞进行流式细胞分析。

Western blot analysis of extracts from HBP-ALL, and Molt4 using Notch1 (D6F11) XP^® Rabbit Ab. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.

使用Notch1 (D6F11) XP^® Rabbit Ab对HBP-ALL和Molt4提取物进行western blot分析。Notch1 全长蛋白（FL）和跨膜/细胞内区域（NTM）如图所示。