| 货号 | 4530T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Notch2 (D67C8) XP® Rabbit mAb detects endogenous levels of total Notch2 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~110 kDa). |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 110, 300 |
| 参考文献 | 1 . Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. 2 . Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. 3 . Schroeter, E.H. et al. (1998) Nature 393, 382-6. 4 . Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35. 5 . McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73. |
Confocal immunofluorescent analysis of 293 cells using Notch2 (D67C8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (Fluorescent DNA dye). 使用Notch2 (D67C8) XP® Rabbit mAb 兔单抗(绿色) 对293细胞进行激光共聚焦荧光分析。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell lines using Notch2 (D67C8) XP® Rabbit mAb. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated. 使用Notch2 (D67C8) XP® Rabbit mAb对多种细胞提取物进行western blot分析。Notch2蛋白的全长（FL）和跨膜/细胞内区域（NTM）如图所示。 |
