| 货号 | 13812T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | MyoD1 (D8G3) XP® Rabbit mAb recognizes endogenous levels of total MyoD1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Myoblast determination protein 1 (MyoD1), also called myogenic factor 3 (Myf3), is a member of the MyoD family of muscle specific bHLH transcription factors (1). This family is responsible for controlling specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types (2,3). Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins (4-6). Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Berkes, C.A. and Tapscott, S.J. (2005) Semin Cell Dev Biol 16, 585-95. 2 . Tapscott, S.J. (2005) Development 132, 2685-95. 3 . Davis, R.L. et al. (1987) Cell 51, 987-1000. 4 . de la Serna, I.L. et al. (2001) Nat Genet 27, 187-90. 5 . Aziz, A. et al. (2010) Epigenetics 5, 691-5. 6 . Guttridge, D.C. (2004) Curr Opin Clin Nutr Metab Care 7, 443-50. 7 . Ge, Y. and Chen, J. (2012) J Biol Chem 287, 43928-35. |
Confocal immunofluorescent analysis of RH30 (positive, left), and HeLa (negative, right) cells using MyoD (D8G3) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from various cell lines using MyoD1 (D8G3) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, 293 and HeLa cells are negative for MyoD1 protein. |
