| 货号 | 13917T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | CD45 (D9M8I) XP® Rabbit mAb recognizes endogenous levels of total CD45 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4). |
| 存放说明 | -20C |
| 计算分子量 | 180 to 240 |
| 参考文献 | 1 . Huntington, N.D. and Tarlinton, D.M. (2004) Immunol Lett 94, 167-74. 2 . Felberg, J. and Johnson, P. (2000) Biochem Biophys Res Commun 271, 292-8. 3 . Kashio, N. et al. (1998) J Biol Chem 273, 33856-63. 4 . Wang, Y. and Johnson, P. (2005) J Biol Chem 280, 14318-24. |
Flow cytometric analysis of 293 cells (blue) and Jurkat cells (green) using CD45 (D9M8I) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used a secondary antibody | |
Confocal immunofluorescent analysis of Jurkat (left) and 293T (right) cells using CD45 (D9M8I) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD45 (D9M8I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and 293 (right) cell pellets using CD45 (D9M8I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using CD45 (D9M8I) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using CD45 (D9M8I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
