| 货号 | 14013T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/IF-IC |
| 目标/特异性 | PC2 (D1E1S) XP® Rabbit mAb recognizes endogenous levels of total PC2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:3200) IF-F (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3). |
| 存放说明 | -20C |
| 计算分子量 | 65-75 |
| 参考文献 | 1 . Scamuffa, N. et al. (2006) FASEB J 20, 1954-63. 2 . Fugère, M. and Day, R. (2005) Trends Pharmacol Sci 26, 294-301. 3 . Seidah, N.G. and Chrétien, M. (1999) Brain Res 848, 45-62. 4 . Rouillé, Y. et al. (1995) Front Neuroendocrinol 16, 322-61. 5 . Steiner, D.F. et al. (1996) Diabetes Metab 22, 94-104. 6 . Scamuffa, N. et al. (2006) FASEB J 20, 1954-63. |
Western blot analysis of extracts from TT, mouse brain, rat brain, and human hypothalamus, using PC2 (D1E1S) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of TT cells (left) and OVCAR8 (right) cells using PC2 (D1E1S) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human stomach using PC2 (D1E1S) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunoprecipitation of PC2 from TT cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PC2 (D1E1S) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PC2 (D1E1S) XP® Rabbit mAb. | |
Western blot analysis of extracts from TT and OVCAR8 cells using PC2 (D1E1S) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded mouse pancreas using PC2 (D1E1S) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human pancreas using PC2 (D1E1S) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded TT (left) and OVCAR8 (right) cell pellets using PC2 (D1E1S) XP® Rabbit mAb. |
