| 货号 | 14037T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F |
| 目标/特异性 | Secretagogin (D4V1Y) XP® Rabbit mAb recognizes endogenous levels of total secretagogin protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:3200) IF-F (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
| 参考文献 | 1 . Kawasaki, H. et al. (1998) Biometals 11, 277-95. 2 . Bastianelli, E. (2003) Cerebellum 2, 242-62. 3 . Wagner, L. et al. (2000) J Biol Chem 275, 24740-51. 4 . Rogstam, A. et al. (2007) Biochem J 401, 353-63. 5 . Bauer, M.C. et al. (2011) Mol Biosyst 7, 2196-204. 6 . Alpár, A. et al. (2012) Cell Signal 24, 378-87. |
Western blot analysis of extracts from TT cells and rat eye using Secretagogin (D4V1Y) XP® Rabbit mAb. | |
Immunoprecipitation of secretagogin from rat eye extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or Secretagogin (D4V1Y) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Secretagogin (D4V1Y) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of rat retina using Secretagogin (D4V1Y) XP® Rabbit mAb (green). Actin filaments were labeled with Dylight™ 554 Phalloidin #13054 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded normal human pancreas using Secretagogin (D4V1Y) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded rat retina using Secretagogin (D4V1Y) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Secretagogin (D4V1Y) XP® Rabbit mAb. |
