| 货号 | 14058T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P |
| 目标/特异性 | B7-H3 (D9M2L) XP® Rabbit mAb recognizes endogenous levels of total B7-H3 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Chapoval, A.I. et al. (2001) Nat Immunol 2, 269-74. 2 . Leitner, J. et al. (2009) Eur J Immunol 39, 1754-64. 3 . Ling, V. et al. (2003) Genomics 82, 365-77. 4 . Petroff, M.G. et al. (2005) Am J Pathol 167, 465-73. 5 . Roth, T.J. et al. (2007) Cancer Res 67, 7893-900. 6 . Loo, D. et al. (2012) Clin Cancer Res 18, 3834-45. 7 . Seaman, S. et al. (2007) Cancer Cell 11, 539-54. 8 . Zang, X. et al. (2010) Mod Pathol 23, 1104-12. |
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded colon carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded LNCaP (left) and NCI-H69 (right) cell pellets using B7-H3 (D9M2L) XP® Rabbit mAb. | |
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H3 (D9M2L) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using B7-H3 (D9M2L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
