| 货号 | 14168T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | Coilin (D2L3J) XP® Rabbit mAb recognizes endogenous levels of total coilin protein. |
| 使用方法 | WB(1:1000) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The nuclear protein coilin (COIL) is found in eukaryotic nucleoplasm and serves a marker for sub-organelles known as Cajal bodies (1,2). Cajal bodies (CB) are nuclear structures that are home to various RNA-processing complexes, including those responsible for pre-mRNA splicing, processing of rRNA and histone pre-mRNA, and telomere maintenance (1-3). The presence of coilin protein is essential for CB formation, and the protein plays a role in maintaining Cajal body structural integrity (4,5). Research studies indicate that coilin binds RNA, including telomerase RNA (hTR), pre-rRNA, and U2 snRNA, in addition to DNA (5). Additional research indicates that coilin protein may exhibit specific RNase activity towards hTR and U2 snRNA transcripts, and that this activity may be regulated through phosphorylation of coilin (6). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Dundr, M. (2012) Curr Opin Cell Biol 24, 415-22. 2 . Lam, Y.W. et al. (2002) Mol Biol Cell 13, 2461-73. 3 . Andrade, L.E. et al. (1991) J Exp Med 173, 1407-19. 4 . Hebert, M.D. (2013) Int J Biochem Cell Biol 45, 1314-7. 5 . Broome, H.J. and Hebert, M.D. (2012) PLoS One 7, e36300. 6 . Broome, H.J. et al. (2013) Biol Open 2, 407-15. |
Confocal immunofluorescent analysis of HeLa cells using Coilin (D2L3J) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from IMR-32, HeLa, and COS-7 cells using Coilin (D2L3J) XP® Rabbit mAb. |
