| 货号 | 14543T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | INPP4b (D9K1B) XP® Rabbit mAb recognizes endogenous levels of total INPP4b protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphotidylinositol lipids and phosphoinositides are important second messengers, their generation controlling many cellular events. Intracellular levels of these molecules are regulated by phosphoinositide kinases and phosphatases. One of the best characterized lipid kinases is phosphoinositide 3-kinase (PI3K), which is responsible for phosphorylation on the D-3 position of the inositide head group (1). This action of PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN, the well characterized partnering phosphatase, reverses this process by removing the phosphate from PI(3,4,5)P3 at the D-3 position to generate PI(4,5)P2 (1,2). Dephosphorylation on the D-5 position to generate PI(3,4)P2 occurs through the action of SHIP1 or SHIP2 (3), and dephosphorylation on the D-4 position to generate PI(3)P can occur through the action of inositol polyphosphate 4-phosphatase isoenzymes type I (INPP4a) and type II (INPP4b) (4,5). While INPP4a has been implicated in neuronal survival and megakaryocyte lineage determination (6,7), less is understood about INPP4b. It has been shown that two splice variants of INPP4b occur in mice, each showing distinct tissue distribution and subcellular localization (5,8). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 3 . Ware, M.D. et al. (1996) Blood 88, 2833-40. 4 . Norris, F.A. et al. (1995) J Biol Chem 270, 16128-33. 5 . Norris, F.A. et al. (1997) J Biol Chem 272, 23859-64. 6 . Nystuen, A. et al. (2001) Neuron 32, 203-12. 7 . Vyas, P. et al. (2000) Proc Natl Acad Sci USA 97, 13696-701. 8 . Ferron, M. and Vacher, J. (2006) Gene 376, 152-61. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using INPP4b (D9K1B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using INPP4b (D9K1B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded normal human breast using INPP4b (D9K1B) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded MCF7 (left) or HeLa (right) cell pellets using INPP4b (D9K1B) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using INPP4b (D9K1B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
