| 货号 | 14572T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P |
| 目标/特异性 | B7-H4 (D1M8I) XP® Rabbit mAb recognizes endogenous levels of total B7-H4 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | B7 homolog 4 (B7-H4, VTCN1) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses (1-3). B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain (3). The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production (1,4,5). Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells (1). The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer (6). Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Sica, G.L. et al. (2003) Immunity 18, 849-61. 2 . Prasad, D.V. et al. (2003) Immunity 18, 863-73. 3 . Zang, X. et al. (2003) Proc Natl Acad Sci U S A 100, 10388-92. 4 . He, C. et al. (2011) Clin Dev Immunol 2011, 695834. 5 . Yi, K.H. and Chen, L. (2009) Immunol Rev 229, 145-51. 6 . Salceda, S. et al. (2005) Exp Cell Res 306, 128-41. 7 . Dangaj, D. and Scholler, N. (2013) Oncoimmunology 2, e25913. 8 . Dangaj, D. et al. (2013) Cancer Res 73, 4820-9. |
Immunohistochemical analysis of paraffin-embedded SK-BR-3 (left) and NCI-H1299 (right) cell pellets using B7-H4 (D1M8I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using B7-H4 (D1M8I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lobular carcinoma of the breast using B7-H4 (D1M8I) XP® Rabbit mAb. | |
Western blot analysis of extracts from SK-BR-3, T-47D, and NCI-H1299 cells using B7-H4 (D1M8I) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H4 (D1M8I) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
