| 货号 | 14569T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Myeloperoxidase (E1E7I) XP® Rabbit mAb recognizes endogenous levels of total myeloperoxidase protein. This antibody recognizes the full-length and heavy chain subunits of human myeloperoxidase protein. |
| 使用方法 | WB(1:1000) IHC-P (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1). |
| 存放说明 | -20C |
| 计算分子量 | 60, 80-90 |
| 参考文献 | 1 . Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625. 2 . Chang, K.S. et al. (1986) Blood 68, 1411-4. 3 . Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55. 4 . Morishita, K. et al. (1987) J Biol Chem 262, 3844-51. 5 . Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61. 6 . Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7. |
Immunohistochemical analysis of paraffin-embedded HL-60 (left) and HeLa (right) cell pellets using Myeloperoxidase (E1E7I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded colon adenocarcinoma using Myeloperoxidase (E1E7I) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using Myeloperoxidase (E1E7I) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Myeloperoxidase (E1E7I) XP® Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). |
