BCL6 (D4I2V) XP® Rabbit mAb【科瑞奥博】

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BCL6 (D4I2V) XP® Rabbit mAb

货号： 14895T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	14895T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IP , F , IF-IC , ChIP
目标/特异性	BCL6 (D4I2V) XP^® Rabbit mAb recognizes endogenous levels of total BCL6 protein. It is predicted to detect both human BCL6 isoform 1 and isoform 2.
使用方法	WB(1:1000) IP (1:200) F (1:400) IF-IC (1:100) ChIP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景
存放说明	-20C
计算分子量	87-98
参考文献	1 . Ye, B.H. et al. (1993) Science 262, 747-50. 2 . Onizuka, T. et al. (1995) Blood 86, 28-37. 3 . Zollman, S. et al. (1994) Proc Natl Acad Sci U S A 91, 10717-21. 4 . Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75. 5 . Shvarts, A. et al. (2002) Genes Dev 16, 681-6. 6 . Moriyama, M. et al. (1997) Oncogene 14, 2465-74. 7 . Niu, H. et al. (1998) Genes Dev 12, 1953-61. 8 . Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13.

参考图片

Confocal immunofluorescent analysis of Ramos (left) and Jurkat (right) cells using BCL6 (D4I2V) XP^® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western blot analysis of extracts from various cell lines using BCL6 (D4I2V) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Flow cytometric analysis of Jurkat cells (blue) and Daudi cells (green) using BCL6 (D4I2V) XP^® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ Raji cells and either 5 µl of BCL6 (D4I2V) XP^® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human HSP90β Promoter Primers #12899 and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunoprecipitation of BCL6 from Ramos cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or BCL6 (D4I2V) XP^® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BCL6 (D4I2V) XP^® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Flow cytometric analysis of Ramos cells (green) and Jurkat cells (blue) using BCL6 (D4I2V) XP^® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.