| 货号 | 4903T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total nanog protein. |
| 使用方法 | WB(1:2000) IHC-P (1:800) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Kim, J. et al. (2008) Cell 132, 1049-61. 2 . Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9. 3 . Yu, J. et al. (2007) Science 318, 1917-20. |
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Nanog (D73G4) XP® 兔源单克隆抗体对NTERA-2 细胞（左）和HeLa细胞（右）进行激光共聚焦荧光分析。用DY-554鬼笔环肽（红色）标记肌动蛋白丝。 | |
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb.使用Nanog (D73G4) XP® 兔源单克隆抗体对HeLa细胞（蓝色）和NTERA-2细胞（绿色）进行流式细胞分析。 | |
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb.使用兔源单克隆抗体Nanog (D73G4) XP® 对石蜡包埋的人精原细胞瘤进行免疫组化分析。 | |
Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb.使用Nanog (D73G4) XP® 兔源单克隆抗体对NCCIT，NTERA-2和iPS细胞提取物进行Western blot分析。 |
