| 货号 | 5030T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | MEF2C (D80C1) XP® Rabbit mAb detects endogenous levels of total MEF2C protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, there are four MEF2C-related genes (MEF2A, MEF2B, MEF2C and MEF2D) that encode proteins that exhibit significant amino acid sequence similarity within their DNA binding domains and, to a lesser extent, throughout the rest of the proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Recently, several groups have reported MEF2 binding activity and MEF2 proteins in a wide variety of cell types where these proteins appear to play an important role in growth factor- and stress-induced early gene responses (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 50-60 |
| 参考文献 | 1 . Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13. 2 . Martin, J.F. et al. (1994) Mol Cell Biol 14, 1647-56. 3 . Yu, Y.T. et al. (1992) Genes Dev 6, 1783-98. 4 . Han, J. et al. (1997) Nature 386, 296-299. 5 . Kato, Y. et al. (1997) EMBO J 16, 7054-66. 6 . Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30. |
Confocal immunofluorescent analysis of C2C12 cells, undifferentiated (left) or differentiated for 3 days (right), using MEF2C (D80C1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测C2C12细胞，左图为未处理的，右图为分化三天后的细胞，检测抗体为MEF2C (D80C1) XP® Rabbit mAb，呈绿色。肌动蛋白丝被DY-554鬼笔环肽标记，呈红色。 | |
Western blot analysis of extracts from various cells lines using MEF2C (D80C1) XP® Rabbit mAb.Western blot检测细胞提取物：各种细胞系，使用的抗体是MEF2C (D80C1) XP® Rabbit mAb。 |
