| 货号 | 8074T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | DR5 (D4E9) XP® Rabbit mAb recognizes endogenous levels of total DR5 protein. This antibody detects both the short and long isoforms of DR5. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. |
| 存放说明 | -20C |
| 计算分子量 | 40, 48 |
| 参考文献 | 1 . Nagata, S. (1997) Cell 88, 355-65. 2 . Thorburn, A. (2004) Cell Signal 16, 139-44. 3 . Wiley, S.R. et al. (1995) Immunity 3, 673-82. 4 . Walczak, H. et al. (1997) EMBO J 16, 5386-97. 5 . Chaudhary, P.M. et al. (1997) Immunity 7, 821-30. 6 . MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20. 7 . Wu, G.S. et al. (2000) Adv Exp Med Biol 465, 143-51. 8 . Wu, G.S. et al. (1997) Nat Genet 17, 141-3. 9 . Finnberg, N. et al. (2005) Mol Cell Biol 25, 2000-13. 10 . Screaton, G.R. et al. (1997) Curr Biol 7, 693-6. |
Western blot analysis of extracts from various cell lines using DR5 (D4E9) XP® Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为DR5 (D4E9) XP® Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from A549 cells, untreated or doxorubicin-treated (500 nM) for the indicated times, using DR5 (D4E9) XP® Rabbit mAb. Western blot 检测A549细胞提取物，未经处理或 doxorubicin 处理(500 nM)一定的时间，使用抗体为DR5 (D4E9) XP® Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with the long isoform of human DR5 (hDR5, +), using DR5 (D4E9) XP® Rabbit mAb. Western blot 检测293T细胞提取物，空质粒 (-) 或人DR5长亚型 (hDR5, +)转染，使用抗体为 DR5 (D4E9) XP® Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of HT-1080 cells using DR5 (D4E9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HT-1080细胞，使用抗体为DR5 (D4E9) XP® Rabbit mAb 兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
