| 货号 | 20292T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , IF-IC |
| 目标/特异性 | Grp94 (D6X2Q) XP® Rabbit mAb recognizes endogenous levels of total Grp94 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help ensure correct protein folding. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role in helping to facilitate folding of a number of secretory proteins to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One way in which Grp94 promotes tumor immunogenicity is its ability to bind to and present tumor-derived peptides as antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit an anti-cancer immune response (8). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925. 2 . Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344. 3 . Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505. 4 . Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322. 5 . Tamura, Y. et al. (1997) Science 278, 117-120. 6 . Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101. 7 . Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215. 8 . Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331. |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Grp94 (D6X2Q) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Grp94 (D6X2Q) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Grp94 (D6X2Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Grp94 (D6X2Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Grp94 (D6X2Q) XP® Rabbit mAb. |
