| 货号 | 5175T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Dog, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | Cofilin (D3F9) XP® Rabbit mAb detects endogenous levels of total cofilin1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
| 参考文献 | 1 . Carlier, M.F. et al. (1999) J. Biol. Chem. 274, 33827-33830. 2 . Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 3 . Arber, S. et al. (1998) Nature 393, 805-809. 4 . Yang, N. et al. (1998) Nature 393, 809-812. 5 . Toshima, J. et al. (2001) J Biol Chem 276, 31449-58. 6 . Nebl, G. et al. (1996) J Biol Chem 271, 26276-80. |
Confocal immunofluorescent analysis of HeLa cells using Cofilin (D3F9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Cofilin (D3F9) XP® Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白（红色）。 | |
Western blot analysis of extracts from various cell types using Cofilin (D3F9) XP® Rabbit mAb.使用Cofilin (D3F9) XP® Rabbit mAb，免疫印迹（Western Blot）分析不同细胞系中Cofilin (D3F9)蛋白水平。 |
