| 货号 | 5318T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F , IF-IC |
| 目标/特异性 | AIF (D39D2) XP® Rabbit mAb detects endogenous levels of total AIF protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7). |
| 存放说明 | -20C |
| 计算分子量 | 67 |
| 参考文献 | 1 . Daugas, E. et al. (2000) FEBS Lett 476, 118-23. 2 . Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50. 3 . Susin, S.A. et al. (1999) Nature 397, 441-6. 4 . Joza, N. et al. (2001) Nature 410, 549-54. 5 . Ye, H. et al. (2002) Nat Struct Biol 9, 680-4. 6 . Miramar, M.D. et al. (2001) J Biol Chem 276, 16391-8. 7 . Klein, J.A. et al. (2002) Nature 419, 367-74. 8 . Kozako, T. et al. (2015) Sci Rep 5, 11345. |
Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP ® Rabbit mAb兔单抗. Western blot检测Jurkat、C2C12和C6细胞系的提取物，使用抗体为AIF (D39D2) XP ® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP ® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析C2C12细胞，使用抗体为AIF (D39D2) XP ® Rabbit mAb兔单抗 #5318 （绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5 ®＃4084（荧光DNA染料）。 | |
Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP™ Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，使用抗体为 AIF (D39D2) XP™ Rabbit mAb兔单抗（绿色），用MitoTracker® Red CMXRos 标记线粒体（红色）进行共定位。蓝色pseudocolor=DRAQ5®＃4084（荧光DNA染料）。 |
