| 货号 | 5324T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P |
| 目标/特异性 | eIF2α (D7D3) XP® Rabbit mAb detects endogenous levels of total eIF2α protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
| 参考文献 | 1 . Kimball, S.R. (1999) Int J Biochem Cell Biol 31, 25-9. 2 . de Haro, C. et al. (1996) FASEB J 10, 1378-87. 3 . Kaufman, R.J. (1999) Genes Dev 13, 1211-33. 4 . Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-8. 5 . Cheshire, J.L. et al. (1999) J Biol Chem 274, 4801-6. 6 . Zamanian-Daryoush, M. et al. (2000) Mol Cell Biol 20, 1278-90. |
Western blot analysis of extracts from various cell lines using eIF2α (D7D3) XP® Rabbit mAb. 使用eIF2α (D7D3) XP® Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF2α (D7D3) XP® Rabbit mAb. 使用eIF2α (D7D3) XP®Rabbit mAb兔单抗，免疫组化分析人类肺癌组织的石蜡切片。 |
