| 货号 | 5332T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-F , IF-IC |
| 目标/特异性 | Desmin (D93F5) XP® Rabbit mAb detects endogenous levels of total desmin protein. |
| 使用方法 | WB(1:1000) IF-F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubules. Major types of intermediate filaments are distinguished and expressed in particular cell types: cytokeratins (epithelial cells), glial fibrillary acidic protein or GFAP (glial cells), desmin (skeletal, visceral and certain vascular smooth muscle cells), vimentin (mesenchyme origin) and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers to distinguish between the two forms of neoplasm (3). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs. The desmin cytoskeleton provides a connection among myofibrils, organelles and the cytoskeleton (4). Desmin knockout mice develop cardiomyopathy, skeletal and smooth muscle defects (5). In humans, desmin related myopathies might be caused by mutations in the corresponding desmin gene or in proteins with which desmin interacts, including αB-crystallin and synemin. Disorganized desmin filaments and the accumulation of protein aggregates comprised predominantly of desmin characterize desmin-related myopathies (reviewed in 6,7). |
| 存放说明 | -20C |
| 计算分子量 | 53 |
| 参考文献 | 1 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 2 . Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93. 3 . Leader, M. et al. (1987) Histopathology 11, 63-72. 4 . Capetanaki, Y. et al. (2007) Exp Cell Res 313, 2063-76. 5 . Li, Z. et al. (1996) Dev Biol 175, 362-6. 6 . Paulin, D. and Li, Z. (2004) Exp Cell Res 301, 1-7. 7 . Paulin, D. et al. (2004) J Pathol 204, 418-27. |
Confocal immunofluorescent analysis of C2C12 cells (left) and mouse heart tissue (right) using Desmin (D93F5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ 5® #4084 (fluorescent DNA dye). 使用Desmin (D93F5) XP® Rabbit mAb (绿色)标记，共聚焦免疫荧光分析C2C12细胞（左图）和小鼠心肌组织（右图）。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from C2C12 cells, rat heart and human heart using Desmin (D93F5) XP® Rabbit mAb. 使用Desmin (D93F5) XP® Rabbit mAb，免疫印迹（Western Blot）分析C2C12细胞、大鼠心肌和人源心肌中Desmin蛋白水平。 |
