HIF-1β/ARNT (D28F3) XP® Rabbit mAb【科瑞奥博】

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HIF-1β/ARNT (D28F3) XP® Rabbit mAb

货号： 5537T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	5537T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , ChIP
目标/特异性	HIF-1β/ARNT (D28F3) XP^® Rabbit mAb detects endogenous levels of total HIF-1β/ARNT protein.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:100) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
存放说明	-20C
计算分子量	87
参考文献	1 . Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. 2 . Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. 3 . Jaakkola, P. et al. (2001) Science 292, 468-72. 4 . Maxwell, P.H. et al. (1999) Nature 399, 271-5. 5 . Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11. 6 . Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. 7 . Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. 8 . Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82. 9 . Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62. 10 . Gunton, J.E. et al. (2005) Cell 122, 337-49.

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ T47D cells treated with BNF (1μM) for 45 min and either 10 μl of HIF-1β/ARNT (D28F3) XP^® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^®Human NFE2L2 Intron 1 Primers #81126, human CYP1A1 promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunohistochemical analysis of paraffin-embedded mouse colon using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.使用HIF-1β/ARNT (D28F3) XP® 兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HIF-1β/ARNT (D28F3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照多肽（左）或抗原特异性多肽（右）存在的条件下使用HIF-1β/ARNT (D28F3) XP®兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化实验。

Western blot analysis of extracts from various cell types using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.使用HIF-1β/ARNT (D28F3) XP® 兔单抗对多种细胞提取物进行western blot实验。