βIG-H3 (D31B8) XP® Rabbit mAb【科瑞奥博】

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βIG-H3 (D31B8) XP® Rabbit mAb

货号： 5601T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	5601T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IP , IF-IC
目标/特异性	βIG-H3 (D31B8) XP® Rabbit mAb detects endogenous levels of total βIG-H3
使用方法	WB(1:1000) IP (1:100) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).
存放说明	-20C
计算分子量	70
参考文献	1 . Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22. 2 . Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84. 3 . Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14. 4 . Kim, J.E. et al. (2003) Oncogene 22, 2045-53. 5 . Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61. 6 . Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9. 7 . Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41. 8 . Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96. 9 . Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9. 10 . Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62. 11 . Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900. 12 . LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9. 13 . Munier, F.L. et al. (1997) Nat Genet 15, 247-51.

参考图片

Western blot analysis of extracts from A549 cells treated with hTGFβ-3 #8425 (100 ng/ml for 12 hours) and OVCAR8 cells using βIG-H3 (D31B8) XP® Rabbit mAb.使用hTGFβ-3 #8425（100 ng/ml 12小时）处理的A549细胞和OVCAR8细胞提取物进行western blot分析，使用βIG-H3 (D31B8) XP®兔单抗进行实验。

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β-1 #8915 (100 ng/ml for 24 hours, right), using βIG-H3 (D31B8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对未处理（左）或hTGF-β-1 #8915 (100 ng/ml 24 小时, 右)处理的A549细胞进行激光共聚焦荧光分析，使用 βIG-H3 (D31B8) XP®兔单抗（绿色）进行实验。肌动蛋白丝使用DY-554鬼笔环肽标记。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。