| 货号 | 5664T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F |
| 目标/特异性 | CNPase (D83E10) Rabbit mAb recognizes endogenous levels of total CNPase protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CNPase (2, 3’-cyclic nucleotide 3-phosphodiesterase) catalyzes the in vitro hydrolysis of 2’, 3’-cyclic nucleotides to produce 2’-nucleotides. The in vivo molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4). |
| 存放说明 | -20C |
| 计算分子量 | 47 |
| 参考文献 | 1 . Esposito, C. et al. (2008) Biochemistry 47, 308-19. 2 . Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8. 3 . Lee, J. et al. (2005) J Cell Biol 170, 661-73. 4 . Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74. |
Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠小脑，使用CNPase (D83E10) XP®Rabbit mAb 兔单抗(绿色）和α/β-Synuclein (Syn205) Mouse mAb 鼠单抗 #2644（红色）进行标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb. Western blot分析多种脑组织提取物，使用的抗体是CNPase (D83E10) XP® Rabbit mAb 兔单抗。 |
