| 货号 | 5679T |
| 描述 | Pdx1 (D59H3) XP® Rabbit mAb detects endogenous levels of total Pdx1 protein. |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F , IF-IC |
| 目标/特异性 | Pdx1 (D59H3) XP® Rabbit mAb detects endogenous levels of total Pdx1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Kaneto, H. et al. (2008) Endocr J 55, 235-52. 2 . Habener, J.F. et al. (2005) Endocrinology 146, 1025-34. 3 . Guz, Y. et al. (1995) Development 121, 11-8. 4 . Ohlsson, H. et al. (1993) EMBO J 12, 4251-9. 5 . Watada, H. et al. (1996) Diabetes 45, 1826-31. 6 . Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34. 7 . Cockburn, B.N. et al. (2004) J Clin Endocrinol Metab 89, 971-8. |
Western blot analysis of extracts from INS-1 cells using Pdx1 (D59H3) XP® Rabbit mAb. Wetern blot检测INS-1细胞提取物，所用抗体为Pdx1 (D59H3) XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of rat pancreas using Pdx1 (D59H3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of INS-1 cells (left) and KNRK cells (right) using Pdx1 (D59H3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测INS-1细胞（左）和KNRK（右）提取物，所用抗体为Pdx1 (D59H3) XP® Rabbit mAb 兔单抗(绿)，微丝用DY-554 phalloidin染色(红色)。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) |
