| 货号 | 5684T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F |
| 目标/特异性 | EAAT1 (D44E2) XP® Rabbit mAb recognizes endogenous levels of total EAAT1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3).EAAT2 accounts for up to 90% of the total glutamate transport in brain while EAAT1 contributes the remaining 5-10% (4). The contribution of EAAT1 in neurotransmission is unclear since EAAT2 is much more abundant. However, EAAT1 expression is upregulated by increasing concentrations of glutamate in the media of cultured primary astrocytes, potentially giving this glutamate transporter additional importance (5). EAAT1 has neuroprotective potential following ischemia since reactive astrocytes and activated microglia express EAAT1 but not EAAT2 (6). |
| 存放说明 | -20C |
| 计算分子量 | 58 |
| 参考文献 | 1 . Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8. 2 . Tanaka, K. et al. (1997) Science 276, 1699-702. 3 . Casado, M. et al. (1993) J Biol Chem 268, 27313-7. 4 . Hediger, M.A. (1999) Am J Physiol 277, F487-92. 5 . Gegelashvili, G. et al. (1996) Neuroreport 8, 261-5. 6 . Beschorner, R. et al. (2007) Histopathology 50, 897-910. |
Confocal immunofluorescent analysis of rat brain using EAAT1 (D44E2) XP® Rabbit mAb (green). Red = Propidium Iodide (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠大脑组织，使用的抗体是EAAT1 (D44E2) XP® Rabbit mAb 兔单抗（绿色）。红色=Propiduim Iodide (荧光DNA染料)。 | |
Western blot analysis of extracts of tissue from mouse brain, rat brain, and human cerebellum using EAAT1 (D44E2) XP® Rabbit mAb. Western blot分析小鼠大脑、大鼠大脑及人小脑提取物，使用的抗体是EAAT1 (D44E2) XP® Rabbit mAb 兔单抗。 |
