| 货号 | 5933T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Hamster,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | DJ-1 (D29E5) XP® Rabbit mAb recognizes endogenous levels of total DJ-1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Parkinsons disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Borrelli, E. (2005) Neuron 45, 479-81. 2 . Bonifati, V. et al. (2003) Science 299, 256-9. 3 . Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13. 4 . Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63. 5 . Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93. 6 . Goldberg, M.S. et al. (2005) Neuron 45, 489-96. |
Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). | |
Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). |
