| 货号 | 6487T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | α-Actinin (D6F6) XP® Rabbit mAb recognizes endogenous levels of total α-actinin protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11. 2 . Nyman-Huttunen, H. et al. (2006) J Cell Sci 119, 3057-66. 3 . Triplett, J.W. and Pavalko, F.M. (2006) Am J Physiol Cell Physiol 291, C909-21. |
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb.用α-Actinin (D6F6) XP®Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of SNB19 cells using α-Actinin (D6F6) XP® Rabbit mAb (green) showing colocalization with actin filaments that were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用α-Actinin (D6F6) XP®Rabbit mAb（绿色）对SNB19细胞进行激光共聚焦免疫荧光分析，同时使用β-Actin (8H10D10) mouse mAb #3700（红色）标记肌动蛋白丝。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye)。 |
