Chk2 (D9C6) XP® Rabbit mAb【科瑞奥博】

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Chk2 (D9C6) XP® Rabbit mAb

货号： 6334T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	6334T
同种亚型	Rabbit IgG
反应种属	Human,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , IF-IC
目标/特异性	Chk2 (D9C6) XP^® Rabbit mAb recognizes endogenous levels of total Chk2 protein.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:400) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).Chk2是哺乳动物中和芽殖酵母Rad53和裂殖酵母Cds1检验点激酶同源的蛋白(1-3)。Chk2的氨基末端结构域包含有一个七丝氨酸或者苏氨酸残基(Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68)组成的系列，每个系列紧跟着谷氨酸(SQ 或TQ模体)。这些地方是已知的ATM/ATR激酶磷酸化的首选作用位点(4,5)。电离辐射(IR)引起DNA损伤之后，UV照射处理或者羟基脲处理，这个区域的68位苏氨酸和其他位点被ATM/ATR磷酸化(5-7)。因此，SQ/TQ簇结构域似乎具有调节功能。第68位苏氨酸的磷酸化是后续激活步骤的先决条件，激活归因于激酶结构域激活回路中Chk2第383位和387位苏氨酸残基的自体磷酸化(8)。
存放说明	-20C
计算分子量	62
参考文献	1 . Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415. 2 . Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665. 3 . Murakami, H. and Okayama, H. (1995) Nature 374, 817-819. 4 . Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86. 5 . Matsuoka, S. et al. (2000) Proc Natl Acad Sci USA 97, 10389-94. 6 . Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. 7 . Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936. 8 . Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

参考图片

Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using Chk2 (D9C6) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋人卵巢浆液性腺癌组织，使用的抗体为Chk2 (D9C6) XP® Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human lung carcioma using Chk2 (D9C6) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋人肺癌组织，使用的抗体为Chk2 (D9C6) XP® Rabbit mAb。

Western blot analysis of extracts from various cell lines using Chk2 (D9C6) XP® Rabbit mAb.Western blot 方法检测不同细胞提取物，使用的抗体为 Chk2 (D9C6) XP® Rabbit mAb。

Immunoprecipitation of Chk2 from 293 cell extracts using Chk2 (D9C6) XP® Rabbit mAb (lane 2). Western blot detection was performed using the same antibody. Lane 1 is 10% input.从293细胞提取物中免疫沉淀Chk2蛋白，使用的抗体为Chk2 (D9C6) XP® Rabbit mAb (泳道2)。使用同一抗体进行western blot检测。

Confocal immunofluorescent analysis of HCT 116 (left) and HCT-15 (right) cells using Chk2 (D9C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测HCT 116细胞（左图）和HCT-15细胞（右图），使用的抗体为Chk2 (D9C6) XP® Rabbit mAb，呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。

Immunohistochemical analysis of paraffin-embedded cell pellets, HCT 116 (left) or MDA-MB-231 (right), using Chk2 (D9C6) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋HCT 116 (左图) 和 MDA-MB-231 (右图)细胞, 使用的抗体为Chk2 (D9C6) XP® Rabbit mAb。