| 货号 | 6997T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC |
| 目标/特异性 | EphA2 (D4A2) XP® Rabbit mAb recognizes endogenous levels of total EphA2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
| 参考文献 | 1 . Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244. 2 . Klein, R. (2001) Curr Opin Cell Biol 13, 196-203. 3 . Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9. 4 . Holder, N. and Klein, R. (1999) Development 126, 2033-44. 5 . Brückner, K. et al. (1997) Science 275, 1640-3. 6 . Palmer, A. et al. (2002) Mol Cell 9, 725-37. 7 . Kalo, M.S. et al. (2001) J Biol Chem 276, 38940-8. 8 . Guo, H. et al. (2006) Cancer Res 66, 7050-8. |
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using EphA2 (D4A2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using EphA2 (D4A2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded SNB19 (left) and SKMEL28 (right) cell pellets using EphA2 (D4A2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of A549 cells (left) and SK-MEL-28 cells (right) using EphA2 (D4A2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 用抗EphA2 的兔源单克隆抗体(D4A2) XP®（绿色）对A549 细胞（左）和SK-MEL-28细胞（右）进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色 | |
Western blot analysis of extracts from various cell lines using EphA2 (D4A2) XP® Rabbit mAb用抗EphA2 的兔源单克隆抗体(D4A2) XP®对各种细胞的提取物进行免疫印迹检测。 |
