| 货号 | 77699T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P |
| 目标/特异性 | CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb recognizes endogenous levels of total CD31 (PECAM-1) protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
| 参考文献 | 1 . Newman, P.J. (1997) J. Clin. Invest. 99, 3-8. 2 . Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72. 3 . Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24. |
Immunohistochemical analysis of paraffin-embedded mouse kidney using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse liver using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A2058 xenograft using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb. | |
Western blot analysis of extracts from 32D (CD31/PECAM-1 positive) and 3T3 (CD31/PECAM-1 negative) cells using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
