LC3A (D50G8) XP® Rabbit mAb【科瑞奥博】

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LC3A (D50G8) XP® Rabbit mAb

货号： 4599T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	4599T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,
来源宿主	Rabbit IgG
应用	WB, IHC-P , F , IF-IC
目标/特异性	LC3A (D50G8) XP® Rabbit mAb detects endogenous levels of total LC3A protein. This antibody may also react with LC3B.
使用方法	WB(1:1000) IHC-P (1:6400) F (1:100) IF-IC (1:400)

性能
供应商	CST
灵敏度	Endogenous
背景	Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11).
存放说明	-20C
计算分子量	14, 16
参考文献	1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4 . Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97. 5 . Lang, T. et al. (1998) EMBO J. 17, 3597-607. 6 . Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28. 7 . He, H. et al. (2003) J. Biol. Chem. 278, 29278-87. 8 . Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10. 9 . Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42. 10 . Ichimura, Y. et al. (2000) Nature 408, 488-92. 11 . Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.

参考图片

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LC3A (D50G8) XP® Rabbit mAb (left) orRabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3A (D50G8) XP® Rabbit mAb.

Western blot分析HeLa和NIH/3T3细胞的提取物，未处理或氯喹处理(50 μM, 过夜)，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。

Flow cytometric analysis of Chloroquine-treated HeLa cells, using LC3A (D50G8) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

流式细胞术分析氯喹处理的HeLa细胞，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗（蓝色）Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）作为对照。

Immunohistochemical analysis of paraffin-embedded human glioblastoma multiforme using LC3A (D50G8) XP® Rabbit mAb.

免疫组化分析石蜡包埋的人多形性胶质母细胞瘤，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。

免疫组化分析石蜡包埋的人结肠癌，使用LC3A (D50G8) XP® Rabbit mAb 兔单抗（左边）或 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（右边）。