| 货号 | 8127T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Rab10 (D36C4) XP® Rabbit mAb recognizes endogenous levels of total Rab10 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . Chen, Y.T. et al. (1993) Proc Natl Acad Sci USA 90, 6508-12. 2 . Burton, J.L. et al. (1994) EMBO J 13, 5547-58. 3 . Roland, J.T. et al. (2009) J Biol Chem 284, 1213-23. 4 . Chen, Y. et al. (2009) Biochem J 422, 229-35. 5 . Schuck, S. et al. (2007) Traffic 8, 47-60. 6 . Babbey, C.M. et al. (2006) Mol Biol Cell 17, 3156-75. 7 . Sano, H. et al. (2007) Cell Metab 5, 293-303. 8 . Larance, M. et al. (2005) J Biol Chem 280, 37803-13. |
Western blot analysis of extracts from various cell lines using Rab10 (D36C4) XP® Rabbit mAb. 对多种细胞系提取物使用Rab10 (D36C4) XP® Rabbit mAb兔单抗进行Western blot分析。 | |
Confocal immunofluorescent analysis of MCF7 cells using Rab10 (D36C4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对MCF7细胞使用Rab10 (D36C4) XP® Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 |
