| 货号 | 8186T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | FoxA2/HNF3β (D56D6) XP® Rabbit mAb recognizes endogenous levels of total FoxA2/HNF3β protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and therefore increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Zhang, L. et al. (2005) Cell Metab 2, 141-8. 2 . Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110. 3 . Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22. 4 . Weinstein, D.C. et al. (1994) Cell 78, 575-88. 5 . Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74. |
Western blot analysis of extracts from Hep G2 cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb. Wetern blot检测Hep G2 细胞系提取物，所用抗体为FoxA2/HNF3β (D56D6) XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of Hep G2 (left) and HeLa (right) cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 激光共聚焦免疫荧光检测Hep G2(左)和HeLa(右)细胞提取物，微丝用DY-554 phalloidin染色(红色)。 |
