| 货号 | 2691T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | PRAS40 (D23C7) XP® Rabbit mAb detects endogenous levels of total PRAS40 protein |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).p>许多生长因子和激素诱导PI3K激酶信号通路，从而激活下游效应蛋白，例如丝氨酸/苏氨酸激酶Akt（1,2）。一个已知的Akt的底物是一个40 kDa的，富含脯氨酸的蛋白（PRAS40），可以结合14-3-3蛋白（2）。PRAS40还能够结合mTOR，传递Akt信号到mTOR复合物。抑制mTOR信号可以刺激PRAS40结合到mTOR上，反过来抑制mTOR的活性（3）。在缺失胰岛素的细胞内PRAS40和mTOR复合物1（mTORC1）中的Raptor 结合，进而抑制细胞周期蛋白Rheb调节的mTORC1信号通路的激活。Akt磷酸化PRAS40的Thr246位点，解除PRAS40对mTORC1的抑制（4）。反过来mTORC1磷酸化PRAS40的Ser183（5）。 |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94. 3 . Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23. 4 . Sancak, Y. et al. (2007) Mol Cell 25, 903-15. 5 . Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39. |
Western blot analysis of extracts from various cell types using PRAS40 (D23C7) XP® Rabbit mAb. Western blot 方法检测各种细胞提取物，使用的抗体是PRAS40 (D23C7) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PRAS40 (D23C7) XP® Rabbit mAb. 免疫组织化学方法检测石蜡包埋的人乳腺癌组织，使用的抗体是PRAS40 (D23C7) XP® Rabbit mAb兔单抗。 |
