GABARAPL1 (D5R9Y) XP® Rabbit mAb
货号：
26632T 基本售价：
1550.0 元 规格：
-
产品信息
概述| 货号 | 26632T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, F , IF-IC |
| 目标/特异性 | GABARAPL1 (D5R9Y) XP® Rabbit mAb recognizes endogenous levels of total GABARAPL1 protein. This antibody does not cross react with other GABARAP family members. |
| 使用方法 | WB(1:1000)
F (1:100)
IF-IC (1:200) |
性能| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) appears to be more highly expressed in the CNS as compared to other family members (12-14). Expression of GABARAPL1 is associated with prognosis of some cancers, including hepatocellular and breast cancer (15,16). Inhibition of GABARAPL1 expression in breast cancer cells attenuates autophagic flux, results in metabolic changes, and leads to cancer promoting activities (17). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
| 参考文献 | 1 . Wang, H. et al. (1999) Nature 397, 69-72.
2 . Shpilka, T. et al. (2011) Genome Biol 12, 226.
3 . Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.
4 . Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24.
5 . Tanida, I. et al. (2004) J Biol Chem 279, 36268-76.
6 . Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50.
7 . Tanida, I. et al. (2001) J Biol Chem 276, 1701-6.
8 . Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
9 . Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505.
10 . Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21.
11 . Vernier-Magnin, S. et al. (2001) Biochem Biophys Res Commun 284, 118-25.
12 . Nemos, C. et al. (2003) Brain Res Mol Brain Res 119, 216-9.
13 . Wang, Y. et al. (2006) Neuroscience 140, 1265-76.
14 . Le Grand, J.N. et al. (2013) PLoS One 8, e63133.
15 . Liu, C. et al. (2014) Oncol Rep 31, 2043-8.
16 . Berthier, A. et al. (2010) Br J Cancer 102, 1024-31.
17 . Boyer-Guittaut, M. et al. (2014) Autophagy 10, 986-1003.
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参考图片Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with chloroquine (50 μM, 24 hr; right), using GABARAPL1 (D5R9Y) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
Western blot analysis of extracts from HeLa, C2C12, and C6 cells, untreated (-) or treated with chloroquine (50 μM, overnight; +), using GABARAPL1 (D5R9Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human GABARAP protein (hGABARAP-Myc/DDK; +), human GABARAPL1 protein (hGABARAPL1-Myc/DDK; +), or human GABARAPL2 protein (hGABARAPL2-Myc/DDK; +) using GABARAPL1 (D5R9Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
Flow cytometric analysis of HeLa cells using GABARAPL1 (D5R9Y) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
