| 货号 | 8685T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , IF-IC , ChIP |
| 目标/特异性 | Smad2/3 (D7G7) XP® Rabbit mAb recognizes endogenous levels of total Smad2/3 protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:1600) ChIP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 52, 60 |
| 参考文献 | 1 . Heldin, C.H. et al. (1997) Nature 390, 465-71. 2 . Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94. 3 . Derynck, R. et al. (1998) Cell 95, 737-740. 4 . Massagué, J. (1998) Annu Rev Biochem 67, 753-91. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Wu, G. et al. (2000) Science 287, 92-7. 7 . Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7. 8 . Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69. |
Western blot analysis of extracts from various cell lines using Smad2/3 (D7G7) XP® Rabbit mAb. 用Smad2/3 (D7G7) XP® Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells, serum starved (left), treated with hTGF-β3 #8425 (100 ng/ml, 30 min, center), or treated with hTGF-β3 and SB43152 (10 ug/mL, 1 hr, right), using Smad2/3 (D7G7) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 用Smad2/3 (D7G7) XP® Rabbit mAb兔单抗 (绿色) 对血清饥饿处理（左），hTGF-β3 #8425 (100 ng/ml, 30 min, 中)处理或hTGF-β3 和SB43152 (10 ug/mL, 1 hr, 右)的HeLa细胞进行激光共聚焦荧光分析。使用DY-554 phalloidin（红色）标记肌动蛋白丝。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HaCaT cells treated with hTGF-β3 #8425 (7 ng/ml, 1 hr) and either 10 μl of Smad2/3 (D7G7) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Intron 1 Primers #4669, SimpleChIP® Human ID1 Promoter Primers #5139, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 用SimpleChIP® Enzymatic Chromatin IP Kit (磁柱) #9003对经过hTGF-β3 #8425 (7 ng/ml, 1 hr)处理的4 x 106 HaCaT细胞和10μl mad2/3 (D7G7) XP®兔单抗或2μl Normal Rabbit IgG #2729进行染色质免疫共沉淀。富集到的DNA与和SimpleChIP® Human CDKN1A Intron1 引物#4669，SimpleChIP® Human ID1 Promoter引物和SimpleChIP®人αSatellite Repeat引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量对比input染色质总量进行相对定量，Input中染色质量设定值为1。 | |
Flow cytometric analysis of HeLa cells using Smad2/3 (D7G7) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用Smad2/3 (D7G7) XP® Rabbit mAb兔单抗（蓝色）和Rabbit (DA1E) mAb IgG XP® 同型对照#3900 (红色)对HeLa细胞进行流式细胞分析。 |
