p38 MAPK (D13E1) XP® Rabbit mAb【科瑞奥博】

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p38 MAPK (D13E1) XP® Rabbit mAb

货号： 8690T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	8690T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Hamster,Monkey,Bovine,Pig,
来源宿主	Rabbit IgG
应用	WB, IHC-P , F , IF-IC
目标/特异性	p38 MAPK (D13E1) XP^® Rabbit mAb recognizes endogenous levels of total p38α, β, or γ MAPK protein. This antibody does not recognize p38δ, SAPK/JNK, or p44/42 MAPK proteins.
使用方法	WB(1:1000) IHC-P (1:400) F (1:800) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).   SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
存放说明	-20C
计算分子量	40
参考文献	1 . Rouse, J. et al. (1994) Cell 78, 1027-1037. 2 . Han, J. et al. (1994) Science 265, 808-11. 3 . Lee, J.C. et al. (1994) Nature 372, 739-46. 4 . Freshney, N.W. et al. (1994) Cell 78, 1039-49. 5 . Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6. 6 . Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4. 7 . Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30. 8 . Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38. 9 . Cuenda, A. et al. (1995) FEBS Lett 364, 229-33. 10 . Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb.

Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP® Rabbit mAb.

Flow cytometric analysis of HeLa cells using p38 MAPK (D13E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p38 MAPK (D13E1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with UV (100 mJ/cm2 with 30 min recovery; right), using p38 MAPK (D13E1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).