| 货号 | 8824T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | Myosin IIb (D8H8) XP® Rabbit mAb recognizes endogenous levels of total myosin IIb protein. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
| 参考文献 | 1 . Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18. 2 . Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83. 3 . Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309. 4 . Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80. 5 . Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68. 6 . Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67. 7 . Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55. |
Western blot analysis of extracts from various cell types using Myosin IIb (D8H8) XP® Rabbit mAb.使用Myosin IIb (D8H8) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of COS-7 cells using Myosin IIb (D8H8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Myosin IIb (D8H8) XP® Rabbit mAb（绿色）对COS-7细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). |
