| 货号 | 8975T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC |
| 目标/特异性 | LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb recognizes endogenous levels of total LAMTOR1/C11orf59 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3 and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Sancak, Y. et al. (2008) Science 320, 1496-501. 2 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4 . Wullschleger, S. et al. (2006) Cell 124, 471-84. 5 . Sancak, Y. et al. (2010) Cell 141, 290-303. |
Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. Western blot 分析不同细胞系提取物，所用抗体为LAMTOR1/C11orf59 (D11H6) XP ® Rabbit mAb兔单抗。 | |
共聚焦免疫荧光分析HeLa细胞，所用抗体为LAMTOR1/C11orf59 (D11H6) XP ® Rabbit mAb 兔单抗(绿色)。肌动蛋白丝采用DY-554 phalloidin标记。蓝色伪彩为DRAQ5 ® #4084 (荧光 DNA 染料)。 | |
Confocal immunofluorescent analysis of HeLa cells using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
