| 货号 | 9042T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | PIAS3 (D5F9) XP® Rabbit mAb recognizes endogenous levels of total PIAS3 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The protein inhibitor of activated Stat (PIAS) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a small ubiquitin-related modifier (SUMO) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (10), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition, PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14). |
| 存放说明 | -20C |
| 计算分子量 | 65-75 |
| 参考文献 | 1 . Liu, B. et al. (1998) Proc Natl Acad Sci USA 95, 10626-31. 2 . Chung, C.D. et al. (1997) Science 278, 1803-5. 3 . Arora, T. et al. (2003) J Biol Chem 278, 21327-30. 4 . Liu, B. et al. (2004) Nat Immunol 5, 891-8. 5 . Schmidt, D. and Müller, S. (2002) Proc Natl Acad Sci USA 99, 2872-7. 6 . Kotaja, N. et al. (2002) Mol Cell Biol 22, 5222-34. 7 . Liu, B. et al. (2005) Mol Cell Biol 25, 1113-23. 8 . Tahk, S. et al. (2007) Proc Natl Acad Sci USA 104, 11643-8. 9 . Bischof, O. et al. (2006) Mol Cell 22, 783-94. 10 . Tolkunova, E. et al. (2007) J Mol Biol 374, 1200-12. 11 . Long, J. et al. (2004) Proc Natl Acad Sci USA 101, 99-104. 12 . Murdoch, R.N. and Edwards, T. (1992) Biochem Int 28, 1029-37. 13 . Liu, B. et al. (2007) Cell 129, 903-14. 14 . Weber, S. et al. (2009) Genes Dev 23, 118-32. |
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PIAS3 siRNA I #9073 (+), or SignalSilence® PIAS3 siRNA II #9031 (+), using PIAS3 (D5F9) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析RD细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PIAS3 siRNA I #9073 (+), 或 SignalSilence® PIAS3 siRNA II #9031 (+),使用抗体是PIAS3 (D5F9) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。PIAS3 (D5F9) XP® Rabbit mAb证实了PIAS3表达的沉默，β-Actin (D6A8) Rabbit mAb用来检测内参。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human PIAS3 (hPIAS3; +), using PIAS3 (D5F9) XP® Rabbit mAb.Western blot分析293T细胞的细胞提取物，对照转染(-)或转染表达全长人源PIAS3(hPIAS3; +)的构建质粒，使用抗体是 PIAS3 (D5F9) XP® Rabbit mAb。 | |
Western blot analysis of extracts from various cell lines using PIAS3 (D5F9) XP® Rabbit mAb.Western blot分析多种细胞系的细胞提取物，使用抗体是PIAS3 (D5F9) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of A-204 (positive, left) and Hep G2 (low expression, right) cells using PIAS3 (D5F9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).共聚焦分析A-204(阳性，左图)和HepG2(低表达，右图)细胞，使用抗体是PIAS3 (D5F9) XP® Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin标记(红色)。 |
