FGF Receptor 1 (D8E4) XP® Rabbit mAb【科瑞奥博】

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FGF Receptor 1 (D8E4) XP® Rabbit mAb

货号： 9740T 基本售价： 1550.0 元规格： -

产品信息

概述
货号	9740T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , F , IF-IC
目标/特异性	FGF Receptor 1 (D8E4) XP® Rabbit mAb detects endogenous levels of total FGF receptor 1 protein. This antibody does not cross-react with other FGF receptor family members.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:500) F (1:400) IF-IC (1:200)

性能
供应商	CST
灵敏度	Endogenous
背景	Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).
存放说明	-20C
计算分子量	92 , 120, 145
参考文献	1 . Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. 2 . Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. 3 . Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. 4 . Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. 5 . Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

参考图片

Flow cytometric analysis of A-204 cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞术方法检测A-204细胞，使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (蓝色)，与浓度相当的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)相比。

Western blot analysis of extracts from A-204 (FGFR1 positive), KG-1a (FGFR1 oncogenic partner-FGFR1 fusion), A172 (FGFR1 low), and HT-29 (FGFR1 negative) cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测A-204细胞(FGFR1阳性), KG-1a细胞 (FGFR1 致癌伴侣-FGFR1融合蛋白), A172 细胞(FGFR1低表达)和HT-29 细胞(FGFR1 阴性)，使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (上图)和 β-Actin (D6A8) Rabbit mAb #8457 (下图).

Confocal immunofluorescent analysis of A204 cells (positive, left), KG-1 cells (positive, middle) and A172 cells (weak expression, right) using FGF Receptor 1 (D8E4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测A204细胞（阳性，左图），KG-1细胞（阳性，中图）和A172细胞（弱表达，右图），使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (绿色)。蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human kidney using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肾脏组织，使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.