Caspase-3 (D3R6Y) Rabbit mAb
货号：
14220T 基本售价：
1350.0 元 规格：
-
产品信息
概述| 货号 | 14220T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB |
| 目标/特异性 | Caspase-3 (D3R6Y) Rabbit mAb recognizes endogenous levels of total caspase-3 protein. This antibody detects full-length caspase-3 as well as the large subunit (p20) of caspase-3 resulting from cleavage during apoptosis. |
| 使用方法 | Western Blotting (1:1000)
|
性能| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 35, 19, 17 |
| 参考文献 | 1 . Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2 . Nicholson, D. W. et al. (1995) Nature 376, 37-43.
|
参考图片Western blot analysis of various cell lines, untreated (-) or treated with Staurosporine #9953 (1 μM; 3 hr) or with Etoposide #2200 (25 μM, overnight), using Caspase-3 (D3R6Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MCF7 cells are negative for caspase-3 expression. |
